Evidence for Two Adhesive Antigens on the K99 Reference Strain Escherichia coli B41

Anionic and cationic components of the K99 surface antigen from Escherichia coli B41.

Succinate Dehydrogenase-dependent Nutritional Requirement for Succinate in Mutants of Escherichia coli ~ 1 2 . By I. T. CREAGHAN and J. R. GUEST Heterothallic Phytophthora : Evidence for Hormonal Regulation of Sexual Reproduction. By W. H. KO Purification and Properties of an NAD(P)+-linked Formaldehyde Dehydrogenase from Methylococcus capsulatus (Bath). By D. I. STIRLING and H. DALTON . A Spec...

Analysis of a naturally occurring K99+ enterotoxigenic Escherichia coli strain that fails to produce K99.

A spontaneously occurring field isolate of enterotoxigenic Escherichia coli that was genotypically K99+ but phenotypically K99- was analyzed for the reason that it did not express K99. The defect, which was cis active, was located within an area 5' to the first gene required for K99 biogenesis and was the result of the deletion of a single base pair.

Confirmed nucleotide sequence of fanF of Escherichia coli K99 fimbriae.

Isoelectric point of cell-free K99 antigen exhibiting hemagglutinating properties.

The isoelectric point of the K99 antigen in partially purified preparations isolated from Escherichia coli B41 was 4.2. Electrofocused K99 antigen hemagglutinated guinea pig and sheep erythrocytes and gave a single precipitin line on diffusion against antisera to E. coli B41 and absorbed factor K99 antisera.

Structural studies on the Escherichia coli O101 lipopolysaccharide found in association with F41 and K99 fimbriae.

In this study it was shown that the O101 lipopolysaccharide isolated from Escherichia coli B41 did not contain an O-specific polysaccharide and that its sugar moiety is probably restricted to the core oligosaccharide. It is characterized by the presence of galactose, glucose, N-acetylglucosamine, heptose and 3-deoxy-D-manno-2-octulosonic acid and the fatty acid composition is typical of an Ente...